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CRISPR@CEDOC: Affordable genome editing of conventional cell lines, iPSCs, and primary cells
We are a research group working at the CEDOC. The group leader has been publishing for several years on the RAG1/RAG2 endonuclease- and Activation-induced deaminase-dependent processes underlying the immunoglobulin gene rearrangements that result in the production of improved antibodies by B cells. As these processes involve the generation of double-strand breaks in the antibody genes, the CRISPR-Cas technology was appealing to us. Like hundreds of groups around the world, we adopted CRISPR-Cas as our preferred gene editing technology to manipulate the genome. In parallel, we also became interested in the technique itself, and we’ve been working on ways to improve it. Given the prohibitive prices charged for genome editing services by companies, we decided to create a service with competitive prices. The service was launched in October 2019. Since then, we initiated more than 30 CRISPR projects for clients from 16 countries. These projects include the generation of Knockouts, single-based knockins, knockins of tags and fluorescent genes, and complex genomic rearrangements are generated using Cas9-gRNA ribonucleoproteins. The target cells include conventional cell lines, induced pluripotent stem cell lines (iPSCs), and primary human or murine cells.
In this presentation, we will introduce the service illustrating our work with the generation by homology directed repair of a cryptic splice-altering variant in the MYBPC3 gene (encoding myosin-binding protein C, cardiac-type), which has been associated with Hypertrophic Cardiomyopathy. I will then tell you about the R&D we have been planning on to optimize some technical procedures.
CRISPR@CEDOC is a highly customizable service operated by working scientists for working scientists. It is also flexible concerning payment arrangements, and it includes the options of a pure service or a collaboration-based interaction.

Nov 24, 2020 12:00 PM in Lisbon

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